Composite

Part:BBa_K3385066:Design

Designed by: Lucas Levassor   Group: iGEM20_DTU-Denmark   (2020-10-16)


T1G3R_ScbhB_glaA


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1858
    Illegal EcoRI site found at 5762
    Illegal EcoRI site found at 7190
    Illegal XbaI site found at 1623
    Illegal XbaI site found at 5793
    Illegal SpeI site found at 1097
    Illegal PstI site found at 893
    Illegal PstI site found at 4024
    Illegal PstI site found at 6600
    Illegal PstI site found at 8334
    Illegal PstI site found at 8935
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1858
    Illegal EcoRI site found at 5762
    Illegal EcoRI site found at 7190
    Illegal NheI site found at 4901
    Illegal SpeI site found at 1097
    Illegal PstI site found at 893
    Illegal PstI site found at 4024
    Illegal PstI site found at 6600
    Illegal PstI site found at 8334
    Illegal PstI site found at 8935
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1858
    Illegal EcoRI site found at 5762
    Illegal EcoRI site found at 7190
    Illegal BglII site found at 3879
    Illegal BglII site found at 5401
    Illegal BglII site found at 8432
    Illegal BamHI site found at 1729
    Illegal BamHI site found at 2157
    Illegal BamHI site found at 4859
    Illegal BamHI site found at 5661
    Illegal BamHI site found at 5787
    Illegal BamHI site found at 6338
    Illegal XhoI site found at 548
    Illegal XhoI site found at 735
    Illegal XhoI site found at 963
    Illegal XhoI site found at 6198
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1858
    Illegal EcoRI site found at 5762
    Illegal EcoRI site found at 7190
    Illegal XbaI site found at 1623
    Illegal XbaI site found at 5793
    Illegal SpeI site found at 1097
    Illegal PstI site found at 893
    Illegal PstI site found at 4024
    Illegal PstI site found at 6600
    Illegal PstI site found at 8334
    Illegal PstI site found at 8935
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1858
    Illegal EcoRI site found at 5762
    Illegal EcoRI site found at 7190
    Illegal XbaI site found at 1623
    Illegal XbaI site found at 5793
    Illegal SpeI site found at 1097
    Illegal PstI site found at 893
    Illegal PstI site found at 4024
    Illegal PstI site found at 6600
    Illegal PstI site found at 8334
    Illegal PstI site found at 8935
    Illegal NgoMIV site found at 2566
    Illegal NgoMIV site found at 5119
    Illegal NgoMIV site found at 8322
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3407
    Illegal BsaI site found at 3545
    Illegal BsaI site found at 4234
    Illegal BsaI.rc site found at 4727
    Illegal SapI site found at 8823
    Illegal SapI site found at 9132


Design Notes

Full construct made using non-overlapping primers, which can pose difficulties on PCR amplification and subsequently USER assembly.

Source

Sequences were obtained using aspgd.org[1] and SingalP-5.0[2].

References

[1] Cerqueira GC, Arnaud MB, Inglis DO, Skrzypek MS, Binkley G, Simison M, Miyasato SR, Binkley J, Orvis J, Shah P, Wymore F, Sherlock G, Wortman JR (2014). The Aspergillus Genome Database: multispecies curation and incorporation of RNA-Seq data to improve structural gene annotations. Nucleic Acids Res 42 (1); D705-10.

[2] SignalP 5.0 improves signal peptide predictions using deep neural networks. José Juan Almagro Armenteros, Konstantinos D. Tsirigos, Casper Kaae Sønderby, Thomas Nordahl Petersen, Ole Winther, Søren Brunak, Gunnar von Heijne and Henrik Nielsen. Nature Biotechnology, 37, 420-423, doi:10.1038/s41587-019-0036-z (2019)